Nonterminal blood sample collection of sufficient volume and quality for research is complicated in mice due to their small size and anatomy. We developed a third method—submental blood collection—which is similar in execution to the submandibular method but with minor changes in animal restraint and collection location. Compared with other techniques, submental collection is easier to perform due to the direct visibility of the target vessels, which are located in a sparsely furred region. Compared with the submandibular method, the submental method did not differ regarding weight change and clotting score but significantly decreased hemolysis and increased the overall number of high-quality samples.
A rapid, simple, and humane method for submandibular bleeding of mice using a lancet
For Use on Mice
Blood Collection. An exception to a Guideline must be described and justified in the Animal Protocol and approved during the normal review process. This document provides direction and guidance on appropriate blood collection methods and volumes for animals used in research at the University of Iowa. These guidelines are intended for use by qualified personnel performing blood collection as described on an IACUC-approved Animal Protocol. There are several factors to consider when determining the appropriate blood collection volume and technique.
Blood Withdrawal II
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When sampling blood from mice, several different techniques can be used, with retroorbital sinus sampling traditionally being the most common. Given the severe tissue trauma caused by retroorbital sampling, alternative methods such as the facial vein route have been developed. The aim of this study was to evaluate 2 techniques for facial vein bleeding in conscious mice to ascertain whether differences in clinical outcomes, practicability of sample collection, and hematologic parameters were apparent. Behavior immediately after sampling was observed, and sample quantity, sampling time, and time until bleeding ceased were measured.